8-Hydroxydeoxyguanosine (8OHdG) is a modified base that
occurs in DNA due to attack by hydroxyl radicals that are formed as
byproducts and intermediates of aerobic metabolism and during oxidative
stress. There is increasing evidence to support the involvement of free
radical reactions in the damage of biomolecules that eventually leads to
several diseases in humans, such as arteriosclerosis, cerebral and heart
ischemia-reperfusion injury, cancer, rheumatoid arthritis, inflammation,
diabetes, aging, and neurodegenerative conditions, such as Alzheimer’s
8OHdG has become increasing popular as a sensitive,
stable and integral marker of oxidative damage in cellular DNA.
Biomonitoring in humans has demonstrated that 8OHdG can be excreted
in the urine, and that a significant increase is caused by exposure to
tobacco smoke and ionizing radiation.
Because 8OHdG is so well correlated with oxidative stress and
damage to DNA, which leads to degenerative disease states, the development
of an antibody that can be used to study DNA damage has numerous
applications. In addition to
the direct study of DNA damage within cells, this antibody has
applications in the development of immunoassays that can monitor 8OHdG
excretion in the urine and serve as a biomarker of oxidative stress.
Industrial uses may extend to the dietary supplement manufacturers,
who could benefit from an immunoassay that could be used to test the
effectiveness of antioxidants and other nutraceuticals.
A goat antiserum to 8-hydroxyguanosine is currently
available. This antiserum has
been shown by ELISA to be completely cross-reactive with
8-hydroxydeoxyguanosine while not cross-reacting with other naturally
occurring nucleotides. This
antibody has also been shown to immunolabel Alzheimer disease neurons using formalin-fixed,
paraffin-embedded tissue sections. This
antibody should be a valuable tool for scientists working to
understand the role of free radical damage in a number of human disease
This antiserum was produced using proprietary methodology whereby the haptin is attached to a carrier that elicits minimal immunoreactivity so that the antiserum has a higher degree of specificity for the haptin. Since there is no overwhelming production of interfering antibodies to the carrier, this antiserum can routinely be used without further purification. Pseud-Immune™ Control Immune Serum (Cat no. GPA018E) from a mock immunized animal is available to be used in conjunction with this antibody.
Yin, B., et al. Free Radic. Biol. Med. 18: 1023-1032, 1995.
|Form:||Whole Serum - liquid|
|Supplied as:||1 ml vials or bulk quantities|
Reported working dilution using neat serum:*
*Various assay conditions require that the optimum working concentrations be determined by serial dilutions of this product.
|Specificity:||8-Hydroxy-2' deoxyguanosine and 8-hydroxyguanosine|
|0.1% Sodium Azide|
|Storage:||Short term: Refrigerate at 4˚ C|
|Long term: Freeze at -20˚ C|
FOR RESEARCH AND MANUFACTURING USE ONLY.
N/D = Not determined.
N/A = Not applicable.
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