1. Immediately after exposure to x-ray film, wash blot or store
refrigerated in TBST or dH2O
until ready to strip.
Wash membrane 2 x 5 minutes in dH2O.
3. Add 15 ml stripping solution.
minutes at room temperature on a rotary shaker.
Wash membrane 2 x 10 minutes in dH2O.
6. Membrane may now be dried and stored at 4ºC in TBST with
5% Acetic Acid (938 ul glacial acetic acid/15 ml dH2O)