Protocol

 

 

STRIPPING WESTERN BLOTS FOR REPROBING

 

1.  Immediately after exposure to x-ray film, wash blot or store

     refrigerated in TBST or dH2O until ready to strip.

2.   Wash membrane 2 x 5 minutes in dH2O.

3.  Add 15 ml stripping solution.

4.  Incubate 20 minutes at room temperature on a rotary shaker.

5.  Wash membrane 2 x 10 minutes in dH2O.

6.  Membrane may now be dried and stored at 4C in TBST with

     preservative.

 

 Stripping Solution

 

 

 

 

 

 5% Acetic Acid (938 ul glacial acetic acid/15 ml dH2O)