Polyclonal Anti-human

            Amyloid Precursor Protein C-terminal Antiserum

Cat. No: GPA010C       

The amyloid precursor protein (APP) is a large membrane protein whose C-terminus projects into the extracellular space. In Alzheimer's disease (AD), the APP is proteolytically cleaved at the N-terminal of Ab by b-secretase (BACE) to release a ~100 kD APPsb protein into the extracellular space.  The remaining 12 kD fragment remains membrane bound where it can be cleaved at its C-terminus by g-secretase (presenilins) to release the insoluble Ab peptide into the extracellular space with the ~8 kD APP C-terminal fragment (CTFb) remaining membrane bound.  The APP is the subject of intensive investigations to determine how this protein is broken down abnormally in AD brains to give rise to Ab, which is present in senile plaques and vessels.

A goat antiserum to a synthetic peptide that corresponds to amino acids 681-695 of the C-terminus of human APP is currently available. This antiserum has been shown to be immunoreactive with the unconjugated immunizing peptide by ELISA.  In western blots itwpe6.jpg (29255 bytes) immunolabels the APP holoprotein. The smaller CTFb is rapidly degraded and not detected on westerns. This antibody should be a valuable tool for scientists working to understand the role of in Alzheimer’s disease.

This antiserum was produced using proprietary methodology whereby the peptide is attached to a carrier that elicits minimal immunoreactivity so that the antiserum has a higher degree of specificity for the peptide.  Since there is no overwhelming production of interfering antibodies to the carrier, this antiserum can routinely be used without further purification.  Pseud-Immune™  control immune serum (Cat no. GPA018E) from a mock immunized animal is available to be used in conjunction with this antibody as well as the immunizing peptide (Cat no. HSP010C), which can be used to neutralize immunoreactivity.

Manufacturing Reference:

Selkoe, DJ, et al.  Proc. Natl. Acad. Sci. USA 85: 7341-7345, 1988.

Lot No: H149I
Host: Goat
Clone: Polyclonal
Immunogen: Peptide (GYENPTYKFFEQMQN
Form: Whole Serum - liquid
Concentration: N/A
Supplied as: 1 ml vials or bulk quantities
Titer: Reported working dilution using neat serum:*
bulletELISA using immunizing peptide: > 1:25,000
bulletIHC using paraffin sections: N/D
bulletIHC using frozen sections: N/D
bulletWestern blots: > 1:5,000

*Various assay conditions require that the optimum working concentrations be determined by serial dilutions of this product.

Specificity: C-terminal amino acid sequence 681-695 of human Amyloid  Precursor Protein

Preservative

        options: None
0.01% Thimerosal
0.1% Sodium Azide
Storage: Short term: Refrigerate at 4˚ C
Long term: Freeze at -20˚ C

FOR RESEARCH AND MANUFACTURING USE ONLY.

N/D = Not determined.

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