Parkin N-terminal Antiserum
Parkin gene was discovered on chromosome 6 and is responsible for an early
onset form of autosomal recessive juvenile Parkinson disease. It shows
moderate similarity to ubiquitin at the N-terminus and the gene is
expressed in various regions of the brain, including the substantia nigra.
Additionally, it is also expressed in other tissues, such as the heart,
testes and skeletal muscle. The
function of this gene product is currently unknown, but it is speculated
that this protein may function in the control of cell growth,
differentiation and development.
A goat antiserum to a synthetic peptide that corresponds to
amino acids 83-97 of the N-terminus of the human parkin protein is
currently available. This antiserum has been shown to be immunoreactive
unconjugated immunizing peptide by ELISA.
This antibody has been shown to label neurons, Lewy bodies and
speroids in Parkinson disease brains using formalin-fixed,
paraffin-embedded sections and it labels the 65 kD Parkin protein in
Western blots. This antibody
should be a valuable tool for scientists working to understand the role of
Parkin in Parkinson’s disease.
This antiserum was produced using proprietary methodology whereby the peptide is attached to a carrier that elicits minimal immunoreactivity so that the antiserum has a higher degree of specificity for the peptide. Since there is no overwhelming production of interfering antibodies to the carrier, this antiserum can routinely be used without further purification. Pseud-Immune™ control immune serum (Cat no. GPA018E) from a mock immunized animal is available to be used in conjunction with this antibody as well as the immunizing peptide (Cat no. HSP012N), which can be used to neutralize immunoreactivity.
Southwest Immunology, Inc., unpublished results.
|Form:||Whole Serum - liquid|
|Supplied as:||1 ml vials or bulk quantities|
Reported working dilution using neat serum:*
*Various assay conditions require that the optimum working concentrations be determined by serial dilutions of this product.
|Specificity:||N-terminal amino acid sequence 83-97 of human Parkin Protein|
|0.1% Sodium Azide|
|Storage:||Short term: Refrigerate at 4˚ C|
|Long term: Freeze at -20˚ C|
FOR RESEARCH AND MANUFACTURING USE ONLY.
N/D = Not determined.
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