Polyclonal Anti-human

 Synphilin-1 C-terminal Antiserum

Cat. No: GPA021C       

Synphilin-1 is a cytoplasmic protein that is enriched in the neurophil of normal human brain.  Although the exact function of synphilin-1 is unknown, it may possibly have a role as a cytoskeletal protein based on the presence of ankyrin-like repeats and an ATP, GTP-binding domain. It has been shown to be novel is its ability to associate in vivo with a-synuclein to form cytoplasmic eosinophilic inclusions.  Additionally, it has been observed to be present in Lewy bodies of Parkinson disease, which are composed of a-synuclein.  Because synphilin-1 is not found in protein aggregates of Alzheimerís disease, it appears to be specific for a-synuclein-containing lesions.  Synphilin-1 may facilitate the aggregation of a-synuclein into Lewy bodies.

A goat antiserum to a synthetic peptide that corresponds to amino acids 829-847 of the C-terminus of the human synphilin-1 is currently available. This antiserum has been shown to be immunoreactive with thewpe46.jpg (29574 bytes) unconjugated immunizing peptide by ELISA. It also labels Lewy bodies in Parkinsonís disease using formalin-fixed, paraffin-embedded sections and it labels a 90 kD in western blots.  This antibody should be a valuable tool for scientists working to understand the role of synphilin-1 in Parkinsonís disease.

wpe48.jpg (12372 bytes)This antiserum was produced using proprietary methodology whereby the peptide is attached to a carrier that elicits minimal immunoreactivity so that the antiserum has a higher degree of specificity for the peptide.  Since there is no overwhelming production of interfering antibodies to the carrier, this antiserum can routinely be used without further purification.  Pseud-Immuneô control immune serum (Cat no. GPA018E) from a mock immunized animal is available to be used in conjunction with this antibody as well as the immunizing peptide (Cat no. HSP021C) which can be used to neutralize immunoreactivity.

Manufacturing Reference:

Southwest Immunology, Inc., unpublished results.

Lot No: H120A1
Host: Goat
Clone: Polyclonal
Immunogen: Peptide (SLELNGEKDKDKGRTLQRT
Form: Whole Serum - liquid
Concentration: N/A
Supplied as: 1 ml vials or bulk quantities
Titer: Reported working dilution using neat serum:*
bulletELISA using immunizing peptide: > 1:10,655
bulletIHC using paraffin sections: > 1:400
bulletIHC using frozen sections: N/D
bulletWestern blots: > 1:2,000

*Various assay conditions require that the optimum working concentrations be determined by serial dilutions of this product.

Specificity: C-terminal amino acid sequence 829-847 of human Synphilin-1 protein.

Preservative

options: None
0.01% Thimerosal
0.1% Sodium Azide
Storage: Short term: Refrigerate at 4˚ C
Long term: Freeze at -20˚ C

FOR RESEARCH AND MANUFACTURING USE ONLY.

N/D = Not determined.

[Home ] [Company] [New Products] [Protocols] [Contact Us]