Synphilin-1 C-terminal Antiserum
Synphilin-1 is a cytoplasmic protein that is enriched in the
neurophil of normal human brain. Although
the exact function of synphilin-1 is unknown, it may possibly have a role
as a cytoskeletal protein based on the presence of ankyrin-like repeats
and an ATP, GTP-binding domain. It has been shown to be novel is its
ability to associate in vivo
with a-synuclein to form cytoplasmic eosinophilic inclusions.
Additionally, it has been observed to be present in Lewy bodies of
Parkinson disease, which are composed of
a-synuclein. Because synphilin-1 is not found in protein aggregates of
Alzheimerís disease, it appears to be specific for
lesions. Synphilin-1 may
facilitate the aggregation of
a-synuclein into Lewy bodies.
A goat antiserum to a synthetic peptide that
corresponds to amino acids 829-847 of the C-terminus of the human
synphilin-1 is currently available. This antiserum has been shown to be
immunoreactive with the unconjugated immunizing peptide by ELISA. It also
labels Lewy bodies in Parkinsonís disease using formalin-fixed,
paraffin-embedded sections and it labels a 90 kD in western blots.
This antibody should be a valuable tool for scientists working to
understand the role of synphilin-1 in Parkinsonís disease.
Southwest Immunology, Inc., unpublished results.
|Form:||Whole Serum - liquid|
|Supplied as:||1 ml vials or bulk quantities|
Reported working dilution using neat serum:*
*Various assay conditions require that the optimum working concentrations be determined by serial dilutions of this product.
|Specificity:||C-terminal amino acid sequence 829-847 of human Synphilin-1 protein.|
|0.1% Sodium Azide|
|Storage:||Short term: Refrigerate at 4˚ C|
|Long term: Freeze at -20˚ C|
FOR RESEARCH AND MANUFACTURING USE ONLY.
N/D = Not determined.
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