Polyclonal Anti-human

 Tau  N-terminal Antiserum

Cat. No: GPA024N       

Tau is a heterogeneous group of proteins that range in molecular weight from 50-67 kdal. They are instrumental in the assembly of microtubules and have been associated with some disease states. In normal brain, tau is localized in the axons of the neurons, but in some neuropathological lesions, it accumulates with in the body of the neuron. Tau proteins are thought to be abnormally phosphorylated in certain disease states and are found associated with the deposits with the neuron. Tau has been immunohistochemically localized to neurofibrillary tangles of Alzheimer's disease (AD).

A goat antiserum to a synthetic peptide that corresponds to amino acids wpeA.jpg (48971 bytes) 1-16 of the N-terminus of the human tau protein is currently available. This antiserum has been shown to be immunoreactive with the unconjugated immunizing peptide by ELISA.   The antibody has been shown to immunolabel neurofibrillary tangles and senile plaques in formalin-fixed, paraffin-embedded Alzheimer's disease brain.  The antiserum has also been shown by immunogold electron microscopy to bind paired helical filaments, as well as immunolabel the tau proteins on western blots.wpe28.gif (64123 bytes) This antibody should be a valuable tool for scientists working to understand the role of tau in Alzheimer’s disease. wpe2A.gif (9988 bytes)

This antiserum was produced using proprietary methodology whereby the peptide is attached to a carrier that elicits minimal immunoreactivity so that the antiserum has a higher degree of specificity for the peptide.  Since there is no overwhelming production of interfering antibodies to the carrier, this antiserum can routinely be used without further purification.  Pseud-Immune™ control immune serum (Cat no. GPA018E) from a mock immunized animal is available to be used in conjunction with this antibody as well as the immunizing peptide (Cat no. HSP024N) which can be used to neutralize immunoreactivity.

Manufacturing Reference:

Goedert, M., et al. Neuron 8:159-168, 1992.

Lot No: L160A
Host: Goat
Clone: Polyclonal
Immunogen: Peptide (MAEPRQEFEVMEDHAG
Form: Whole Serum - liquid
Concentration: N/A
Supplied as: 1 ml vials or bulk quantities
Titer: Reported working dilution using neat serum:*
bulletELISA using immunizing peptide: > 1:36,450
bulletIHC using paraffin sections: > 1:200
bulletIHC using frozen sections: N/D
bulletWestern blots: > 1:4,000

*Various assay conditions require that the optimum working concentrations be determined by serial dilutions of this product.

Specificity: N-terminal amino acid sequence 1-16 of human Tau protein.


        options: None
0.01% Thimerosal
0.1% Sodium Azide
Storage: Short term: Refrigerate at 4˚ C
Long term: Freeze at -20˚ C


N/D = Not determined.

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