Tau is a heterogeneous group of proteins that range in molecular weight from 50-67 kdal. They are instrumental in the assembly of microtubules and have been associated with some disease states. In normal brain, tau is localized in the axons of the neurons, but in some neuropathological lesions, it accumulates with in the body of the neuron. Tau proteins are thought to be abnormally phosphorylated in certain disease states and are found associated with the deposits with the neuron. Tau has been immunohistochemically localized to neurofibrillary tangles of Alzheimer's disease (AD).

A goat antiserum to a synthetic peptide that corresponds to amino acids 1-16 of the N-terminus of the human tau protein is currently available. This antiserum has been shown to be immunoreactive with the unconjugated immunizing peptide by ELISA.   The antibody has been shown to immunolabel neurofibrillary tangles and senile plaques in formalin-fixed, paraffin-embedded Alzheimer's disease brain.  The antiserum has also been shown by immunogold electron microscopy to bind paired helical filaments, as well as immunolabel the tau proteins on western blots. This antibody should be a valuable tool for scientists working to understand the role of tau in Alzheimer’s disease.

This antiserum was produced using proprietary methodology whereby the peptide is attached to a carrier that elicits minimal immunoreactivity so that the antiserum has a higher degree of specificity for the peptide.  Since there is no overwhelming production of interfering antibodies to the carrier, this antiserum can routinely be used without further purification.  Pseud-Immune™ control immune serum (Cat no. GPA018E) from a mock immunized animal is available to be used in conjunction with this antibody as well as the immunizing peptide (Cat no. HSP024N) which can be used to neutralize immunoreactivity.

Manufacturing Reference:

Goedert, M., et al. Neuron 8:159-168, 1992.