is a 76 amino acid protein that is highly conserved. It is found is all plant and animal species and is produced
in response to stress. The
formation of ubiquitin-protein conjugates is a signal for the selective
degradation of proteins. Ubiquitin
has been immunohistochemically localized to a number of pathological
inclusions. These lesions include Lewy bodies of Parkinson's disease,
neurofibrillary tangles of Alzheimer's disease, Pick bodies of Pick's
disease, Mallory bodies of alcoholic liver disease, cytoplasmic bodies of
a specific myopathy, and Rosenthal fibers within astrocytes.
A rabbit antiserum to a purified preparation of human ubiquitin protein is currently available. This antiserum has been shown to be immunoreactive with the purified protein by ELISA. This antiserun reacts immunohistochemically with ubiquitin-containing lesions in formalin-fixed, paraffin-embedded tissue. This antiserum is especially useful for the immunohistochemical identification of Lewy bodies, which are not well differentiated by histochemical stains. It will also immunolabel ubiquitin on protein blots. This antibody should be a valuable tool for scientists working to understand the role of ubiquitin in various neurological diseases.
Sparkman, D.R., et al. Prep. Biochem. 21:93-104, 1991.
|Form:||Whole Serum - liquid|
|Supplied as:||1 ml vials or bulk quantities|
Reported working dilution using neat serum:*
*Various assay conditions require that the optimum working concentrations be determined by serial dilutions of this product.
|0.1% Sodium Azide|
|Storage:||Short term: Refrigerate at 4˚ C|
|Long term: Freeze at -20˚ C|
FOR RESEARCH AND MANUFACTURING USE ONLY.
N/D = Not determined.
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