Polyclonal Anti-human

 Ubiquitin Antiserum

Cat. No: RPA004EC       

Ubiquitin is a 76 amino acid protein that is highly conserved.  It is found is all plant and animal species and is produced in response to stress.  The formation of ubiquitin-protein conjugates is a signal for the selective degradation of proteins.  Ubiquitin has been immunohistochemically localized to a number of pathological inclusions. These lesions include Lewy bodieswpe4C.jpg (51429 bytes) of Parkinson's disease, neurofibrillary tangles of Alzheimer's disease, Pick bodies of Pick's disease, Mallory bodies of alcoholic liver disease, cytoplasmic bodies of a specific myopathy, and Rosenthal fibers within astrocytes.

A rabbit antiserum to a purified preparation of human ubiquitin protein is currently available.  This antiserum has been shown to be immunoreactive wpe4E.jpg (14433 bytes) with the purified protein by ELISA. This antiserun reacts immunohistochemically with ubiquitin-containing lesions in formalin-fixed, paraffin-embedded tissue. This antiserum is especially useful for the immunohistochemical identification of Lewy bodies, which are not well differentiated by histochemical stains. It will also immunolabel ubiquitin on protein blots.  This antibody should be a valuable tool for scientists working to understand the role of ubiquitin in various neurological diseases.

Manufacturing Reference:

Sparkman, D.R., et al.  Prep. Biochem. 21:93-104, 1991.

Lot No: G038I
Host: Rabbit
Clone: Polyclonal
Immunogen: Human ubiquitin 
Form: Whole Serum - liquid
Concentration: N/A
Supplied as: 1 ml vials or bulk quantities
Titer: Reported working dilution using neat serum:*
bulletELISA using immunizing peptide: > 1:5,000
bulletIHC using paraffin sections: > 1:200
bulletIHC using frozen sections: N/D
bulletWestern blots: > 1:1,000

*Various assay conditions require that the optimum working concentrations be determined by serial dilutions of this product.

Specificity: Human ubiquitin


        options: None
0.01% Thimerosal
0.1% Sodium Azide
Storage: Short term: Refrigerate at 4˚ C
Long term: Freeze at -20˚ C


N/D = Not determined.

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