Ubiquitin Carboxyl-Terminal Hydrolase L1 N-terminal Antiserum
Ubiquitin carboxyl-terminal hydrolase L1 (UCH ĖL1) is one
of the most abundant proteins in the brain and represents 1-2% of total
soluble brain proteins. UCH-L1
activity is thought to cleave polymeric ubiquitin to monomers and to
hydrolyze bonds between ubiquitin-protein conjugatges to regenerate
ubiquitin monomers. In
many degenerative diseases, ubiquitin has been identified as a component
of several inclusion bodies and certain of these are associated with
UCH-L1 activity. One such
inclusion is the Lewy body of Parkinsonís disease.
Since a polymorphism has been detected in the UCH-L1 gene, it is
suspected to have a role is some forms of Parkinsonís disease.
A goat antiserum to a synthetic peptide that corresponds to
amino acids 46-63 of the N-terminus of the human UCH-L1 is currently
available. This antiserum has been shown to be immunoreactive with the
unconjugated immunizing peptide by ELISA. It also labels a 27 kD protein
in western blots. This
antibody should be a valuable tool for scientists working to understand
the role of UCH-L1 in Parkinsonís disease.
This antiserum was produced using proprietary methodology whereby the peptide is attached to a carrier that elicits minimal immunoreactivity so that the antiserum has a higher degree of specificity for the peptide. Since there is no overwhelming production of interfering antibodies to the carrier, this antiserum can routinely be used without further purification. Pseud-Immuneô control immune serum (Cat no. GPA018E) from a mock immunized animal is available to be used in conjunction with this antibody as well as the immunizing peptide (Cat no. HSP016N) which can be used to neutralize immunoreactivity.
Southwest Immunology, Inc., unpublished results.
|Form:||Whole Serum - liquid|
|Supplied as:||1 ml vials or bulk quantities|
Reported working dilution using neat serum:*
*Various assay conditions require that the optimum working concentrations be determined by serial dilutions of this product.
|Specificity:||N-terminal amino acid sequence 46-63 of human Ubiquitin Carboxyl-Terminal Hydrolase L1 protein.|
|0.1% Sodium Azide|
|Storage:||Short term: Refrigerate at 4˚ C|
|Long term: Freeze at -20˚ C|
FOR RESEARCH AND MANUFACTURING USE ONLY.
N/D = Not determined.
[Home ] [Company] [New Products] [Protocols] [Contact Us]