Polyclonal Anti-human

 Ubiquitin Carboxyl-Terminal Hydrolase L1 N-terminal Antiserum

Cat. No: GPA016N       

Ubiquitin carboxyl-terminal hydrolase L1 (UCH ĖL1) is one of the most abundant proteins in the brain and represents 1-2% of total soluble brain proteins.  UCH-L1 activity is thought to cleave polymeric ubiquitin to monomers and to hydrolyze bonds between ubiquitin-protein conjugatges to regenerate ubiquitin monomers.   In many degenerative diseases, ubiquitin has been identified as a component of several inclusion bodies and certain of these are associated with UCH-L1 activity.  One such inclusion is the Lewy body of Parkinsonís disease.  Since a polymorphism has been detected in the UCH-L1 gene, it is suspected to have a role is some forms of Parkinsonís disease.

A goat antiserum to a synthetic peptide that corresponds to amino acids 46-63 of the N-terminus of the human UCH-L1 is currently available. This antiserum has been shown to be immunoreactive with the unconjugated immunizing peptide by ELISA. It also labels a 27 kD protein in western blots.  This antibody should be a valuable tool for scientists working to understand the role of UCH-L1 in Parkinsonís disease.

This antiserum was produced using proprietary methodology whereby the wpe50.jpg (10671 bytes) peptide is attached to a carrier that elicits minimal immunoreactivity so that the antiserum has a higher degree of specificity for the peptide.  Since there is no overwhelming production of interfering antibodies to the carrier, this antiserum can routinely be used without further purification.  Pseud-Immuneô control immune serum (Cat no. GPA018E) from a mock immunized animal is available to be used in conjunction with this antibody as well as the immunizing peptide (Cat no. HSP016N) which can be used to neutralize immunoreactivity.

Manufacturing Reference:    

Southwest Immunology, Inc., unpublished results.

Lot No: J019JB
Host: Goat
Clone: Polyclonal
Immunogen: Peptide (NFRKKQIEELKGQEVSPK
Form: Whole Serum - liquid
Concentration: N/A
Supplied as: 1 ml vials or bulk quantities
Titer: Reported working dilution using neat serum:*
bulletELISA using immunizing peptide: > 1:71,350
bulletIHC using paraffin sections: N/D
bulletIHC using frozen sections: N/D
bulletWestern blots: > 1:2,000

*Various assay conditions require that the optimum working concentrations be determined by serial dilutions of this product.

Specificity: N-terminal amino acid sequence 46-63 of human Ubiquitin Carboxyl-Terminal Hydrolase L1 protein.

Preservative

        options: None
0.01% Thimerosal
0.1% Sodium Azide
Storage: Short term: Refrigerate at 4˚ C
Long term: Freeze at -20˚ C

FOR RESEARCH AND MANUFACTURING USE ONLY.

N/D = Not determined.

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